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| Year:
2016 |
Month:
January
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Volume:
5 |
Issue:
1 |
Page:
12 - 16 |
Detection of ß-lactamase Mediated
Resistance among Pseudomonas
aeruginosa Isolates from Ocular Infections
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Correspondence
Palanisamy Manikandan,
Dr. Palanisamy Manikandan,
Department of Microbiology and Molecular Biology, Aravind Eye Hospital and Postgraduate Institute of Ophthalmology, Avinashi road, Coimbatore-641014, Tamilnadu, India.
E-mail: manikandanpalanisamy@gmail.com :
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Introduction: P.aeruginosa is the most prevalent gram-negative organism in ocular infections. Production of extended spectrum of b-lactamases (ESBL) by them may require alteration in the management protocol of gram-negative ocular infections.
Aim: To analyze the prevalence and detection of ß-lactamase producing P.aeruginosa from ocular infections.
Materials and Methods: This study included all the patients with ocular infections who visited a tertiary care eye hospital in Coimbatore, for a period of four months. During this study period, the isolates were confirmed as P.aeruginosa by standard identification protocol. Minimum inhibitory concentration (MIC) of the antibiotics was determined by agar dilution method.Inducible ß-lactamases and extended spectrum ß-lactamases were detected by double disc diffusion method.
Results: Of the 2644 ocular specimens collected from 2618 patients, a total of 34 isolates of P.aeruginosa were obtained. MICs of cefotaxime and ceftazidime for all the isolates revealed that, 21 (62%) isolates were resistant to cefotaxime. A total of 11 strains were induced to produce ß-lactamase with disc approximation at 20 mm apart and the presence of ESBL was detected in 4.3% of strains through 20 mm disc approximation, 34.7% of strains through 15 mm disc approximation. The synergic effect of cefotaxime with aminoglycosides was seen among 61.7% of strains.
Conclusion: Resistance of P.aeruginosa to b-lactam antibiotics seems to be prevalent in this part of the state. Combination of cefotaxime with aminoglycosides may be more useful for treatment of severe eye infections caused by P. aeruginosa.
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